Real-time detection of mRNA splicing variants with specifically designed reverse-transcription loop-mediated isothermal amplification

Real - Time Detection of HIV - 2 by Reverse Transcription , Loop - Mediated 1 Isothermal Amplification

Real-Time Detection of HIV-2 by Reverse Transcription, Loop-Mediated 1 Isothermal Amplification 2 3 Kelly A. Curtis*, Philip Niedzwiedz, Ae S. Youngpairoj, Donna L. Rudolph, and S. Michele 4 Owen 5 6 Laboratory Branch, Division of HIV/ AIDS Prevention, National Center for HIV/AIDS, 7 Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA, 8 30333 9 10 The fin...

Rapid Detection of West Nile Virus for Loop-Mediated Isothermal Amplification Real-Time Reverse Transcription

Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus.

A one-step, single tube, real-time accelerated reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detecting the envelope gene of West Nile (WN) virus. The RT-LAMP assay is a novel method of gene amplification that amplifies nucleic acid with high specificity, efficiency, and rapidity under isothermal conditions with a set of six specially designed pri...

Real-Time Detection of HIV-2 by Reverse Transcription-Loop-Mediated Isothermal Amplification.

Currently, there are no FDA-approved nucleic acid amplification tests (NAATs) for the detection or confirmation of HIV-2 infection. Here, we describe the development of a real-time assay for the detection of HIV-2 DNA and RNA using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) and the ESEQuant tube scanner, a portable isothermal amplification/detection device.

Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay.

The development and validation of a one-step, real-time, and quantitative dengue virus serotype-specific reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay targeting the 3' noncoding region for the rapid detection and differentiation of dengue virus serotypes are reported. The RT-LAMP assay is very simple and rapid, wherein the amplification can be obtained in 30 min u...